Approximately 20 of plant transcripts have an upstream orf uorf in the 5 utr.
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In plants nmd identifies a stop codon as a ptc and initiates the rapid degradation of the transcript if the 3 untranslated region utr is unusually long or if it harbors an intron.
A cell permeable tetrahydroquinoxalinyl thiophenecarboxylate compound that inhibits nonsense mediated mrna decay nmd in a dose dependent manner and enhances the stability of premature termination codon ptc mutated p53 mrna in n417 and hdqp 1 cells.
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Nmd also regulates the expression of many wild type genes.
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However it does not affect the stability of wild type p53 in u2os cells.
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Nonsense mediated mrna decay nmd is a translation coupled mechanism that eliminates mrnas containing premature translation termination codons ptcs.